The Phospho E-Cadherin (S838/S840) Rabbit Polyclonal Antibody (CABP1074) is a specialized antibody designed for research involving phosphorylated forms of the E-Cadherin protein. E-Cadherin is a crucial cell adhesion molecule involved in maintaining tissue integrity and regulating cell-cell interactions. This antibody, raised in rabbits, is highly specific for the phosphorylated S838/S840 sites of E-Cadherin in human samples, making it an essential tool for studying the regulation and function of this important protein.By targeting the phosphorylated sites of E-Cadherin, this antibody allows for the detection and analysis of E-Cadherin activity in various cell types and experimental conditions. This makes it ideal for research in fields such as cell biology, cancer biology, and developmental biology, where E-Cadherin plays a critical role in cell signaling and tissue organization.
The phosphorylation of E-Cadherin at S838/S840 has been linked to the regulation of cell adhesion and migration, as well as the development and progression of cancer. By using this antibody, researchers can investigate the specific role of phosphorylated E-Cadherin in these processes, providing valuable insights into the mechanisms underlying disease states and potential therapeutic targets.Overall, the Phospho E-Cadherin (S838/S840) Rabbit Polyclonal Antibody (CABP1074) is a powerful tool for research aimed
Cell junction, Cell membrane, Endosome, Golgi apparatus, Single-pass type I membrane protein, trans-Golgi network.
Calculated MW:
97kDa
Observed MW:
125kDa
This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16.
Purification Method:
Affinity purification
Gene ID:
999
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-E-Cadherin-S838/S840 Rabbit pAb (CABP1074) at 1:1000 dilution.HeLa cells were treated by Pervanadate (1 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
