Based on the information in the provided URL, we have the Phospho-DUSP1/DUSP4 (S296/318) Antibody (PAC06189) which is a valuable tool for studying phosphorylation events in the DUSP1 and DUSP4 proteins. This antibody, raised in rabbits, is highly specific and reactive to human samples, making it ideal for use in Western blot applications. The phosphorylation of DUSP1 and DUSP4 at the S296/318 sites plays a crucial role in regulating the activity of these phosphatases, which are known for their role in MAPK signaling and immune response regulation. By targeting these phosphorylation sites, researchers can gain insights into the intricate mechanisms of cellular signaling pathways and immune regulation.
The Phospho-DUSP1/DUSP4 (S296/318) Antibody (PAC06189) is a valuable tool for researchers studying signal transduction pathways, inflammation, and cancer biology. Its specificity and sensitivity make it an essential component in any laboratory conducting research in these fields. By utilizing this antibody, scientists can uncover novel insights into the role of DUSP1 and DUSP4 phosphorylation in health and disease, paving the way for potential therapeutic interventions targeting these pathways.
Synthesized peptide derived from human MKP-1/2 around the phosphorylation site of S296/318.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western Blot analysis of various cells using Phospho-MKP-1/2 (S296/318) Polyclonal Antibody.
Western Blot analysis of JK cells using Phospho-MKP-1/2 (S296/318) Polyclonal Antibody.
Synonyms:
DUSP1; CL100; MKP1; PTPN10; VH1; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; Mitogen-activated protein kinase phosphatase 1; MAP kinase phosphatase 1; MKP-1; Protein-tyrosine phosphatase CL100; DUSP4;
UniProt Protein Function:
MKP-1: a non-receptor, dual-specificity phosphoprotein phosphatase (DUSP). Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP1 specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation. It is induced in human skin fibroblasts by oxidative/heat stress and growth factors. Contains 1 rhodanese domain.
UniProt Protein Details:
Protein type:Protein phosphatase, dual-specificity; EC 3.1.3.48; Motility/polarity/chemotaxis; EC 3.1.3.16
Chromosomal Location of Human Ortholog: 5q34
Cellular Component: cytoplasm; nucleus
Molecular Function:protein tyrosine/threonine phosphatase activity; protein binding; non-membrane spanning protein tyrosine phosphatase activity; protein tyrosine/serine/threonine phosphatase activity; MAP kinase tyrosine/serine/threonine phosphatase activity
Biological Process: negative regulation of MAP kinase activity; response to light stimulus; response to retinoic acid; response to cAMP; negative regulation of MAPKKK cascade; positive regulation of apoptosis; response to glucocorticoid stimulus; negative regulation of meiotic cell cycle; response to testosterone stimulus; protein amino acid dephosphorylation; response to estradiol stimulus; cellular response to hormone stimulus; regulation of apoptosis; response to hydrogen peroxide; endoderm formation; response to oxidative stress; response to calcium ion; negative regulation of apoptosis; inactivation of MAPK activity
NCBI Summary:
The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation. [provided by RefSeq, Jul 2008]
