The Phospho-DPYSL2 (T514) Polyclonal Antibody (CABP0821) is a valuable tool for researchers studying DPYSL2, a protein involved in neuronal development and synaptic function. This antibody, produced in rabbits, is highly specific for phosphorylated DPYSL2 at threonine 514 and is suitable for use in Western blot applications with human samples.DPYSL2, also known as collapsin response mediator protein 2 (CRMP2), is essential for axonal growth and guidance in the nervous system. Phosphorylation of DPYSL2 at threonine 514 is known to regulate its function in cell signaling pathways, making this antibody an important tool for investigating the role of DPYSL2 in neuronal development and neurodegenerative diseases.
By enabling researchers to detect and analyze phosphorylated DPYSL2 in various cell types, this antibody is ideal for studies in neuroscience and neurology. Understanding the regulation of DPYSL2 phosphorylation can provide insights into the mechanisms underlying neurological disorders and may lead to the development of novel therapeutic strategies targeting DPYSL2 signaling pathways.
This gene encodes a member of the collapsin response mediator protein family. Collapsin response mediator proteins form homo- and hetero-tetramers and facilitate neuron guidance, growth and polarity. The encoded protein promotes microtubule assembly and is required for Sema3A-mediated growth cone collapse, and also plays a role in synaptic signaling through interactions with calcium channels. This gene has been implicated in multiple neurological disorders, and hyperphosphorylation of the encoded protein may play a key role in the development of Alzheimer's disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
1808
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-CRMP2/DPYSL2-T514 Rabbit pAb (CABP0821) at 1:1000 dilution or CRMP2/DPYSL2 antibody (CAB14570). SH-SY5Y cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. PC-12 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
