The Phospho-Cytokeratin 8 (KRT8) Polyclonal Antibody (CABP1299) is a valuable tool for researchers studying the phosphorylation of Cytokeratin 8, a protein involved in maintaining the structural integrity of epithelial cells. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.Cytokeratin 8 is phosphorylated at serine 74, a post-translational modification that may play a role in regulating the function of this protein in various cellular processes. By targeting the phosphorylated form of Cytokeratin 8, researchers can gain insights into how its phosphorylation status influences cell behavior and function in normal and disease states.
This antibody allows for the detection and analysis of phosphorylated Cytokeratin 8 in a variety of cell types, making it an important tool for studies in cell biology, cancer research, and other fields where the phosphorylation of this protein may be relevant. By understanding the role of phosphorylated Cytokeratin 8, researchers can potentially uncover new therapeutic targets for diseases involving aberrant cell signaling pathways.
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
3856
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Hep G2, using Phospho-Cytokeratin 8 (KRT8)-S74 Rabbit pAb (CABP1299) at 1:1000 dilution.Hep G2 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
