The Phospho-Cytokeratin 18 (KRT18 S33) Antibody (CABP0263) is a valuable tool for researchers studying cytokeratin 18 phosphorylated at serine 33. This antibody, developed against human samples, is raised in rabbits and has been validated for use in various applications, including Western blotting. By specifically binding to the phosphorylated form of cytokeratin 18, this antibody enables the detection and analysis of this protein modification in different cell types.Cytokeratin 18, a member of the cytoskeletal intermediate filament family, is involved in maintaining the structural integrity of epithelial cells. Phosphorylation of cytokeratin 18 at serine 33 has been linked to various cellular processes, including cell migration, apoptosis, and epithelial cell signaling.
Understanding the role of phospho-cytokeratin 18 in these pathways can provide insights into its function in normal cellular processes and disease states, such as cancer progression.Researchers interested in investigating the impact of phospho-cytokeratin 18 in cellular physiology and disease mechanisms will find the CABP0263 antibody to be a valuable tool for their studies. Its specificity for the phosphorylated form of cytokeratin 18 allows for accurate and reliable analysis, making it suitable for use in immunology, cancer research, and other areas of biomedicine.
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
3875
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HT29 cells untreated or treated with EGF using Phospho-Cytokeratin 18 (KRT18)-S33 Rabbit pAb (CABP0263).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
