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Phospho-Cyclin E1-T77 Rabbit Monoclonal Antibody (CABP1014)

SKU:
CABP1014
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Cell Cycle
€419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-Cyclin E1-T77 Rabbit Monoclonal Antibody
SKU:CABP1014
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Mouse
Immunogen:A synthetic phosphorylated peptide around T77 of human Cyclin E1 (P24864).
Sequence:IPTP D
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:CCNE; pCCNE1; Phospho-Cyclin E1-T77
Positive Sample:NIH/3T3
Conjugate:Unconjugated
Cellular Localization:Nucleus
Calculated MW:47kDa
Observed MW:48kDa

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB.

Purification Method:Affinity purification
Gene ID:898
Clone Number:ARC1559
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of NIH/3T3 cells, using Phospho-Cyclin E1-T77 Rabbit mAb (CABP1014) at 1:1000 dilution.NIH/3T3 cells were treated by Hydroxyurea (4 Mm) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.

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