The Phospho-Cyclin E1 (T77) Rabbit Monoclonal Antibody (CABP1014) is a cutting-edge tool for researchers studying the regulation of cell cycle progression. This antibody specifically targets the phosphorylated form of Cyclin E1 at threonine 77, a critical site involved in cell cycle control. Raised in rabbits, this monoclonal antibody exhibits high reactivity and specificity towards human samples, making it a reliable tool for Western blot applications. By detecting and visualizing the phosphorylated form of Cyclin E1, researchers can gain valuable insights into cell cycle dynamics and potential therapeutic targets for diseases such as cancer.
With its ability to identify and quantify phosphorylated Cyclin E1 in various cell types, the CABP1014 antibody is essential for studies in molecular biology, oncology, and drug development. By understanding the role of Cyclin E1 phosphorylation in cell proliferation and tumorigenesis, researchers can pave the way for innovative treatments and interventions in the field of cancer research.
Product Name:
Phospho-Cyclin E1-T77 Rabbit Monoclonal Antibody
SKU:
CABP1014
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
A synthetic phosphorylated peptide around T77 of human Cyclin E1 (P24864).
Sequence:
IPTP D
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
CCNE; pCCNE1; Phospho-Cyclin E1-T77
Positive Sample:
NIH/3T3
Conjugate:
Unconjugated
Cellular Localization:
Nucleus
Calculated MW:
47kDa
Observed MW:
48kDa
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB.
Purification Method:
Affinity purification
Gene ID:
898
Clone Number:
ARC1559
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Cyclin E1-T77 Rabbit mAb (CABP1014) at 1:1000 dilution.NIH/3T3 cells were treated by Hydroxyurea (4 Mm) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
