The Phospho-CTNNB1 (S552) Polyclonal Antibody (CABP0579) is a valuable tool for researchers studying the phosphorylation of CTNNB1, a key protein involved in cell adhesion and signaling pathways. This antibody, produced in rabbits, has high specificity for detecting phosphorylated CTNNB1 at serine 552 in human samples, making it especially useful for Western blot applications.CTNNB1, also known as beta-catenin, plays a crucial role in Wnt signaling, cell proliferation, and differentiation. Phosphorylation of CTNNB1 at serine 552 has been implicated in regulating its activity and stability, impacting downstream gene expression and cellular processes. By targeting this specific phosphorylation site, researchers can better understand the molecular mechanisms underlying cell signaling and development.
The Phospho-CTNNB1 (S552) Polyclonal Antibody is an essential tool for investigating the role of CTNNB1 phosphorylation in various biological processes, including embryonic development, tissue homeostasis, and cancer progression. Its high reactivity and specificity make it a reliable option for researchers studying the intricate signaling pathways controlled by CTNNB1 and its phosphorylation status. Unlock the potential of your research with this antibody and explore the complex world of cell signaling and regulation.
Product Name:
Phospho-β-Catenin-S552 Rabbit Polyclonal Antibody
SKU:
CABP0579
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S552 of human beta Catenin (NP_001091679).
Cell junction, Cell membrane, Cytoplasm, Nucleus, adherens junction, centrosome, cytoskeleton, microtubule organizing center, spindle pole.
Calculated MW:
85kDa
Observed MW:
106kDa
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1499
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Phospho-beta Catenin-S552 antibody (CABP0579) at 1:1000 dilution. C2C12 cell lysates were treated by CIP at 37°C for 1 hour. NIH/3T3 cells were treated by Forskolin (30uM) for 30 minutes after serum-starvation overnight or treated by CIP at 37°C for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.
