The Phospho-CTNNB1 (S45) Polyclonal Antibody (CABP0580) is a valuable tool for researchers studying the role of CTNNB1 (β-Catenin) phosphorylation in cellular processes such as cell growth, differentiation, and development. This antibody, raised in rabbits, is highly specific for the phosphorylated form of CTNNB1 at Serine 45 and is validated for use in Western blot and immunohistochemistry applications.CTNNB1 is a key signaling molecule in the Wnt pathway, regulating gene expression and cell fate decisions. Phosphorylation of CTNNB1 at Serine 45 is known to modulate its activity, leading to changes in target gene expression and cellular responses.
Understanding the regulation of CTNNB1 phosphorylation is essential for unraveling its complex role in development, tissue homeostasis, and disease progression.With its high specificity and sensitivity, the Phospho-CTNNB1 (S45) Polyclonal Antibody is a valuable tool for investigating the intricate regulation of CTNNB1 phosphorylation and its impact on cellular signaling pathways. This antibody enables researchers to explore the functional significance of CTNNB1 phosphorylation in various biological contexts, making it an invaluable asset for studies in developmental biology, cancer research, and beyond.
Product Name:
Phospho-β-Catenin-S45 Rabbit Polyclonal Antibody
SKU:
CABP0580
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S45 of human CTNNB1 (NP_001091679).
Cell junction, Cell membrane, Cytoplasm, Nucleus, adherens junction, centrosome, cytoskeleton, microtubule organizing center, spindle pole.
Calculated MW:
85kDa
Observed MW:
92kDa
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1499
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of HeLa cells, using Phospho-β-Catenin-S45 antibody (CABP0580) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
