The Phospho-CTNNB1 (S33/S37/T41) Polyclonal Antibody (CABP0524) is a powerful tool for researchers studying the phosphorylation of CTNNB1, a key player in the Wnt signaling pathway. This antibody, generated in rabbits, has high specificity for human samples and has been validated for use in various applications, including Western blotting.CTNNB1, also known as beta-catenin, is a critical component in the Wnt signaling pathway, which plays a crucial role in embryonic development, cell differentiation, and tumorigenesis. Phosphorylation of CTNNB1 at serine 33, serine 37, and threonine 41 regulates its stability and activity, affecting downstream gene expression and cell fate decisions.
The Phospho-CTNNB1 (S33/S37/T41) Polyclonal Antibody allows for the detection and analysis of phosphorylated CTNNB1 in various cell types, providing valuable insights into the role of this protein in signaling pathways and disease processes. Its specificity and reliability make it an essential tool for researchers investigating the molecular mechanisms underlying Wnt signaling and its implications in cancer, developmental biology, and regenerative medicine.
Cell junction, Cell membrane, Cytoplasm, Nucleus, adherens junction, centrosome, cytoskeleton, microtubule organizing center, spindle pole.
Calculated MW:
85kDa
Observed MW:
Refer to figures
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1499
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-β-Catenin-S33/S37/T41 Rabbit pAb (CABP0524) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
