The Phospho-CSNK2A1 (T360/S362) Polyclonal Antibody (CABP0335) is a specialized tool for researchers studying the phosphorylation of CSNK2A1, a key protein involved in various cellular processes. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it suitable for applications such as Western blotting.CSNK2A1, also known as casein kinase 2 alpha 1, is a serine/threonine kinase that plays a crucial role in regulating cell growth, proliferation, and survival. Phosphorylation of CSNK2A1 at threonine 360 and serine 362 is known to impact its activity and function, making the CABP0335 antibody an essential tool for studying these regulatory mechanisms.
Researchers can utilize this antibody to investigate the phosphorylation status of CSNK2A1 in various cell types and experimental conditions, providing valuable insights into its role in cancer, cell signaling, and other biological processes. By understanding the intricate regulation of CSNK2A1 phosphorylation, researchers may uncover new therapeutic targets for diseases related to dysregulated cell growth and proliferation.
Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythm. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. Multiple transcript variants encoding different protein isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
1457
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from 293F cells using Phospho-Casein Kinase 2 alpha (CSNK2A1)-T360/S362 Rabbit pAb (CABP0335) at 1:400 dilution. 293F cells were treated by ERK2 (100ng/ml) at 37℃ for 30 minutes.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime: 60s.
