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Phospho-CSNK1A1/CSNK1A1L (Tyr294) Antibody (PACO24388)

SKU:
PACO24388
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
WB
IHC
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
  • Phospho-CSNK1A1/CSNK1A1L Tyr294 Antibody PACO24388
  • Phospho-CSNK1A1/CSNK1A1L Tyr294 Antibody PACO24388
€549
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Description

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Phospho-CSNK1A1/CSNK1A1L (Tyr294) Antibody (PACO24388)

The Phospho-CSNK1A1/CSNK1A1L (Tyr294) Antibody (PAC024388) is a valuable tool for researchers studying the phosphorylation status of CSNK1A1/CSNK1A1L, proteins involved in cellular signaling pathways. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the phosphorylated form of CSNK1A1/CSNK1A1L at Tyrosine 294, this antibody allows for the detection and analysis of phosphorylation events in various cell types.CSNK1A1/CSNK1A1L are serine/threonine kinases known to play crucial roles in regulating diverse cellular processes, including cell proliferation, differentiation, and apoptosis.

Phosphorylation at Tyr294 has been implicated in modulating the activity of these kinases, influencing downstream signaling cascades. The ability to study this phosphorylation event provides insights into the regulatory mechanisms governing cellular responses and can aid in the development of targeted therapies for diseases where dysregulation of kinase activity is implicated.Researchers in the fields of cell biology, signal transduction, and cancer biology will find the Phospho-CSNK1A1/CSNK1A1L (Tyr294) Antibody (PAC024388) to be a valuable asset in their studies. Its specificity for the phosphorylated form of

Antibody Name:Phospho-CSNK1A1/CSNK1A1L (Tyr294) Antibody (PACO24388)
Antibody SKU:PACO24388
Size:100ul
Host Species:Rabbit
Tested Applications:ELISA, WB, IHC
Recommended Dilutions:ELISA:1:2000-1:10000, WB:1:500-1:1000, IHC:1:50-1:100
Species Reactivity:Human, Mouse, Rat
Immunogen:Peptide sequence around phosphorylation site of tyrosine 294 (Y-D-Y(p)-T-F) derived from Human CK-1 α .
Form:Liquid
Storage Buffer:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Product ImageWestern blot analysis of extracts from HT-29 cells treated with heat shock using CK-1alpha (Phospho-Tyr294) Antibody.The lane on the right is treated with the antigen-specific peptide.
Product ImageImmunohistochemical analysis of paraffin-embedded human brain tissue using CK-1 alpha (Phospho-Tyr294) antibody (left)or the same antibody preincubated with blocking peptide (right).
Background:

Casein kinases are operationally defined by their preferential utilization of acid, c proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.

Synonyms:KC1A; CKI-alpha; CSNK1A1; α isoform; Casein kinase I
UniProt Protein Function:CK1A: an ubiquitous protein kinase of the CK1 family. Participates in Wnt signaling. Phosphorylates beta catenin, marking it for beta-TRCp-mediated ubiquitinylation and proteasomal degradation.
UniProt Protein Details:

Protein type:Protein kinase, CK1; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; CK1 group; CK1 family

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: beta-catenin destruction complex; centrosome; cytosol; keratin filament; membrane; mRNA cleavage and polyadenylation specificity factor complex; nuclear speck

Molecular Function:kinase activity; protein binding; protein kinase activity; protein serine/threonine kinase activity

Biological Process: cell surface receptor linked signal transduction; Golgi organization and biogenesis; intermediate filament cytoskeleton organization and biogenesis; peptidyl-serine phosphorylation; phagocytosis; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; protein amino acid phosphorylation; regulation of cell shape; Wnt receptor signaling pathway

UniProt Code:P48729
NCBI GenInfo Identifier:31077177
NCBI Gene ID:1452
NCBI Accession:P48729.2
UniProt Secondary Accession:P48729,Q4JJA0, Q5U046, Q5U047, Q6FGA2, Q71TU5, Q96HD2 Q9UDK3, D3DQG0, D3DQG1,
UniProt Related Accession:P48729
Molecular Weight:37,567 Da
NCBI Full Name:Casein kinase I isoform alpha
NCBI Synonym Full Names:casein kinase 1 alpha 1
NCBI Official Symbol:CSNK1A1  
NCBI Official Synonym Symbols:CK1; CK1a; CKIa; HLCDGP1; PRO2975; HEL-S-77p  
NCBI Protein Information:casein kinase I isoform alpha
UniProt Protein Name:Casein kinase I isoform alpha
UniProt Synonym Protein Names:CK1
Protein Family:Casein kinase
UniProt Gene Name:CSNK1A1  
UniProt Entry Name:KC1A_HUMAN
AntibodiesELISA Kits
Phospho-CSNK1A1/CSNK1A1L (Y294) Antibody (PACO02492)Casein Kinase I alpha (Phospho-Tyr294) Colorimetric Cell-Based ELISA Kit
Secondary Antibody
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)
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