The Phospho-CSF1R (Y723) Rabbit Polyclonal Antibody (CABP1075) is a valuable tool for researchers studying the CSF1R protein, a receptor involved in cell growth and differentiation. This antibody, generated in rabbits, specifically recognizes the phosphorylated form of CSF1R at tyrosine 723 and is suitable for Western blot applications in human samples.CSF1R, also known as colony-stimulating factor 1 receptor, plays a key role in regulating the development and function of macrophages, which are critical components of the immune system.
Phosphorylation at tyrosine 723 is known to be important for the activation of downstream signaling pathways involved in cell proliferation and survival.By using this antibody, researchers can investigate the phosphorylation status of CSF1R in different cell types and under various conditions, leading to a better understanding of its role in normal physiology and disease states. This antibody is a valuable tool for studies in immunology, cancer research, and other fields where CSF1R signaling is of interest.
Product Name:
Phospho-CD115/CSF-1R-Y723 Polyclonal Antibody
SKU:
CABP1075
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around Y723 of human CSF1R (NP_005202.2).
Cell membrane, Single-pass type I membrane protein.
Calculated MW:
108kDa
Observed MW:
142kDa
The protein encoded by this gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in this gene have been associated with a predisposition to myeloid malignancy. The first intron of this gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Alternative splicing results in multiple transcript variants. Expression of a splice variant from an LTR promoter has been found in Hodgkin lymphoma (HL), HL cell lines and anaplastic large cell lymphoma.
Purification Method:
Affinity purification
Gene ID:
1436
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and 293T transfected with Phospho-CD115/CSF-1R-Y723, using Phospho-CD115/CSF-1R-Y723 (CABP1075) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
