The Phospho-CSF1R (Y708) Polyclonal Antibody (CABP0609) is a valuable tool for researchers studying CSF1R, a receptor tyrosine kinase protein involved in cell growth and differentiation. This antibody, generated in rabbits, specifically targets the phosphorylated Y708 site on the CSF1R protein, allowing for accurate detection and analysis in various experimental settings, particularly Western blot applications.CSF1R plays a crucial role in regulating the development and function of macrophages, immune cells that play a key role in various physiological and pathological processes. Phosphorylation of the Y708 residue of CSF1R is associated with its activation and downstream signaling pathways, making this antibody an important resource for investigating the functions and mechanisms of CSF1R in cellular processes.
By utilizing the Phospho-CSF1R (Y708) Polyclonal Antibody (CABP0609), researchers can enhance their understanding of CSF1R signaling and its impact on cellular pathways related to immune response, inflammation, and cancer development. This antibody is recommended for studies focused on unraveling the complex roles of CSF1R in health and disease, providing valuable insights that may contribute to the development of novel therapeutic strategies targeting CSF1R-mediated pathways.
Product Name:
Phospho-CSF1R-Y708 Rabbit Polyclonal Antibody
SKU:
CABP0609
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around Y708 of human CSF1R (NP_005202.2).
Cell membrane, Single-pass type I membrane protein.
Calculated MW:
108kDa
Observed MW:
175kDa
The protein encoded by this gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in this gene have been associated with a predisposition to myeloid malignancy. The first intron of this gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Alternative splicing results in multiple transcript variants. Expression of a splice variant from an LTR promoter has been found in Hodgkin lymphoma (HL), HL cell lines and anaplastic large cell lymphoma.
Purification Method:
Affinity purification
Gene ID:
1436
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from K-562 cells, using Phospho-CD115/CSF-1R-Y708 Rabbit pAb (CABP0609) at 1:1000 dilution.K-562 cells were treated by G-CSF (25 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
