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Phospho-CSF1R-Y708 Rabbit Polyclonal Antibody (CABP0609)

SKU:
CABP0609
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
€419
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Description

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Product Name:Phospho-CSF1R-Y708 Rabbit Polyclonal Antibody
SKU:CABP0609
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around Y708 of human CSF1R (NP_005202.2).
Sequence:KKYV R
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:FMS; CSFR; FIM2; GPSC; HDLS; C-FMS; CD115; HDLS1; CSF-1R; BANDDOS; M-CSF-R; Phospho-CD115/CSF-1R-Y708
Positive Sample:K-562
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Single-pass type I membrane protein.
Calculated MW:108kDa
Observed MW:175kDa

The protein encoded by this gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in this gene have been associated with a predisposition to myeloid malignancy. The first intron of this gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Alternative splicing results in multiple transcript variants. Expression of a splice variant from an LTR promoter has been found in Hodgkin lymphoma (HL), HL cell lines and anaplastic large cell lymphoma.

Purification Method:Affinity purification
Gene ID:1436
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from K-562 cells, using Phospho-CD115/CSF-1R-Y708 Rabbit pAb (CABP0609) at 1:1000 dilution.K-562 cells were treated by G-CSF (25 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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