The CryAB Polyclonal Antibody (CAB13696) is a valuable tool for researchers studying CryAB, a small heat shock protein known for its role in protecting cells from stress-induced damage. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the CryAB protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for investigations in cell biology, neurology, and cardiovascular research.
CryAB, also known as alpha-B crystallin, is a key player in cellular stress responses and has been implicated in diseases such as neurodegenerative disorders and cardiac dysfunction. By studying the function and regulation of CryAB, researchers can gain insights into these conditions and potentially identify new therapeutic targets. The CryAB Polyclonal Antibody is a valuable tool for advancing our understanding of CryAB biology and its implications for human health.
Product Name:
CRYAB Rabbit Polyclonal Antibody
SKU:
CAB13696
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-175 of human CRYAB (NP_001876.1).
Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1410
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CRYAB Rabbit pAb (CAB13696) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
