The Phospho-CRKL (Y207) Polyclonal Antibody (CABP0824) is a valuable tool for researchers studying CRKL, a signaling protein involved in cellular processes such as cell growth, differentiation, and migration. This antibody, produced in rabbits, exhibits high specificity for phosphorylated CRKL at tyrosine 207 and is validated for use in Western blot experiments.CRKL is a key regulator of various signaling pathways, including those mediated by growth factors and cytokines. Phosphorylation at tyrosine 207 is known to modulate CRKL's activity, influencing its interactions with downstream signaling molecules.
Understanding the role of phospho-CRKL in cellular signaling is important for unraveling its impact on cell behavior and its potential implications in diseases such as cancer.Researchers interested in unraveling the intricate signaling cascades involving CRKL and its phosphorylation events can rely on this antibody to detect and analyze phospho-CRKL levels in experimental samples. Its high reactivity with human samples makes it an ideal choice for studies in cancer biology, cell signaling, and developmental biology. Unlock the potential of phospho-CRKL research with the CABP0824 antibody.
Product Name:
Phospho-CRKL-Y207 Rabbit Polyclonal Antibody
SKU:
CABP0824
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around Y207 of human CRKL (NP_005198.1).
Sequence:
HAYA Q
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB,1:500 - 1:2000 IHC-P,1:50 - 1:100
Synonyms:
CRKL; Phospho-CRKL-Y207
Positive Sample:
K-562
Conjugate:
Unconjugated
Cellular Localization:
cytosol, extrinsic component of postsynaptic membrane, neuromuscular junction, nucleoplasm
Calculated MW:
34kDa
Observed MW:
38kDa
This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.
Purification Method:
Affinity purification
Gene ID:
1399
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from K-562 cells, using Phospho-CRKL-Y207 Rabbit pAb (CABP0824) at 1:1000 dilution or CRKL antibody (CAB11735). K-562 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
