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Phospho-CRK/CRKII-Y221 Rabbit Polyclonal Antibody (CABP0822)

SKU:
CABP0822
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
€419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-CRK/CRKII-Y221 Rabbit Polyclonal Antibody
SKU:CABP0822
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around Y221 of human CRK/CRKII (NP_058431.2).
Sequence:GPYA Q
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:p38; CRKII; Phospho-CRK/CRKII-Y221
Positive Sample:K-562,K-562+CIP
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm.
Calculated MW:34kDa
Observed MW:38kDa

This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.

Purification Method:Affinity purification
Gene ID:1398
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from K562 cells, using Phospho-CRK/CRKII-Y221 Rabbit pAb (CABP0822) at 1:1000 dilution. K562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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