The Phospho-Crk (Y221) Polyclonal Antibody (CABP0822) is a valuable tool for researchers studying Crk, a protein involved in cellular signaling pathways and implicated in cancer progression. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. Crk is a crucial adaptor protein that mediates signal transduction in response to various extracellular stimuli, playing a key role in cell proliferation, migration, and survival. The phosphorylation of Crk at tyrosine 221 (Y221) is known to modulate its activity, affecting downstream signaling events.
By targeting the phosphorylated form of Crk at Y221, researchers can gain insights into the regulation of cellular processes and potentially identify novel therapeutic targets for cancer treatment. This antibody enables the detection and analysis of phosphorylated Crk in various cell types, making it a valuable tool for studies in cancer biology and signaling pathways. Understanding the role of phosphorylated Crk in cancer development and progression can provide valuable insights into the mechanisms underlying this disease, potentially leading to the development of targeted therapies for improved patient outcomes.
Product Name:
Phospho-CRK/CRKII-Y221 Rabbit Polyclonal Antibody
SKU:
CABP0822
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around Y221 of human CRK/CRKII (NP_058431.2).
Sequence:
GPYA Q
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
p38; CRKII; Phospho-CRK/CRKII-Y221
Positive Sample:
K-562,K-562+CIP
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Cytoplasm.
Calculated MW:
34kDa
Observed MW:
38kDa
This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.
Purification Method:
Affinity purification
Gene ID:
1398
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from K562 cells, using Phospho-CRK/CRKII-Y221 Rabbit pAb (CABP0822) at 1:1000 dilution. K562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
