The Phospho-CREB1 (S133) Polyclonal Antibody (CABP0333) is a valuable tool for researchers studying the phosphorylation of CREB1 at serine 133, a critical event in the activation of this transcription factor. This antibody, raised in rabbits, is highly specific to the phosphorylated form of CREB1 and has been validated for use in Western blot applications with human samples.Phosphorylation of CREB1 at serine 133 is known to play a key role in various physiological processes, including neuronal plasticity, memory formation, and response to stress. Dysregulation of CREB1 phosphorylation has been implicated in a range of diseases, making this antibody an essential tool for investigations in neuroscience, cancer research, and drug development.
Researchers can use the Phospho-CREB1 (S133) Polyclonal Antibody to detect and quantify levels of phosphorylated CREB1 in different cell types and tissues, providing valuable insights into the signaling pathways and molecular mechanisms underlying cellular responses to various stimuli. This antibody is a valuable asset for studies aiming to uncover the role of CREB1 phosphorylation in health and disease, ultimately contributing to the development of new therapeutic strategies targeting this critical transcription factor.
Product Name:
Phospho-CREB1-S133 Rabbit Polyclonal Antibody
SKU:
CABP0333
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S133 of human CREB1 (NP_604391.1).
Sequence:
RPSY R
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB,1:500 - 1:1000 IHC-P,1:50 - 1:100
Synonyms:
CREB; CREB-1; Phospho-CREB1-S133
Positive Sample:
NIH/3T3
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
35kDa
Observed MW:
40kDa
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
1385
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-CREB1-S133 Rabbit pAb (CABP0333) at 1:1000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.NIH/3T3 cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
