The Phospho-CREB1 (S129) Polyclonal Antibody (CABP0903) is a valuable tool for researchers studying CREB1, a transcription factor involved in cellular signaling pathways and gene regulation. This antibody, developed using rabbit immunogens, exhibits high reactivity with human samples and is suitable for use in Western blot applications. By specifically binding to the phosphorylated form of CREB1 at serine 129, this antibody enables precise detection and analysis of CREB1 activation in various cell types.CREB1 plays a crucial role in mediating cellular responses to various stimuli, including stress, growth factors, and neurotransmitters.
Phosphorylation at serine 129 is known to regulate CREB1 activity, impacting gene expression and cell function. Understanding the phosphorylation status of CREB1 can provide valuable insights into signaling pathways involved in processes such as neuronal plasticity, learning, and memory. This antibody is an essential tool for researchers investigating the role of CREB1 in neurological disorders, cognitive function, and other physiological responses.
Product Name:
Phospho-CREB1-S129 Rabbit Polyclonal Antibody
SKU:
CABP0903
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S129 of human CREB1 (NP_604391.1).
Sequence:
ILSR R
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:100 - 1:500
Synonyms:
CREB; CREB-1; Phospho-CREB1-S129
Positive Sample:
293T,C6
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
35kDa
Observed MW:
43kDa
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
1385
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using Phospho-CREB1-S129 Rabbit pAb (CABP0903) at 1:500 dilution.293T cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
