The Phospho-CREB1 (S129) Polyclonal Antibody is a valuable tool for researchers studying CREB1, a transcription factor that plays a crucial role in various cellular processes, including neuronal plasticity, learning, and memory. This antibody, developed and produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.Phosphorylation of CREB1 at serine 129 is known to regulate its activity and impact gene expression, making this antibody particularly useful for investigating signaling pathways that involve CREB1 activation.
By targeting this specific phosphorylation site, researchers can analyze the phosphorylation status of CREB1 in various cell types and conditions, providing valuable insights into its role in cellular function and disease processes.With its high specificity and sensitivity, the Phospho-CREB1 (S129) Polyclonal Antibody is a reliable tool for studying the complex mechanisms involving CREB1 and its phosphorylation events. Its applications in neuroscience, cancer research, and other fields make it a versatile reagent for investigating the functions of CREB1 in health and disease.
Product Name:
Phospho-CREB1-S129 Polyclonal Antibody
SKU:
CABP1437
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic phosphorylated peptide around S129 of human CREB1.
Sequence:
ILSR R
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:100 - 1:500
Synonyms:
CREB; CREB-1; Phospho-CREB1-S129
Positive Sample:
293T+λ-PP,C6+λ-PP
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
36KDa
Observed MW:
37kDa
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
1385
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of 293T, using Phospho-CREB1-S129 Rabbit pAb (CABP1437) at 1:400 dilution.293T cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
