The Phospho-Cofilin-1 (Y139) Polyclonal Antibody (CABP0330) is a valuable tool for researchers studying cell motility, cytoskeleton dynamics, and signaling pathways. This antibody, raised in rabbits, specifically targets phosphorylated cofilin-1 at the Y139 site, allowing for precise detection and analysis in various cell types.Cofilin-1 is a key regulator of actin dynamics, playing a crucial role in cell migration, adhesion, and division. Phosphorylation of cofilin-1 at the Y139 site influences its activity, impacting cellular processes such as cytoskeletal rearrangements and cell movement. By targeting this specific phosphorylation site, researchers can gain insights into the regulation of cofilin-1 and its functional implications.
The Phospho-Cofilin-1 (Y139) Polyclonal Antibody is validated for use in Western blot applications, enabling researchers to monitor changes in phosphorylated cofilin-1 levels under different experimental conditions. Its high reactivity with human samples makes it a versatile tool for studies in cell biology, cancer research, and neurobiology. With its ability to detect and quantify phosphorylated cofilin-1, this antibody is essential for advancing our understanding of cellular processes and disease mechanisms.
Product Name:
Phospho-CFL1-Y139 Polyclonal Antibody
SKU:
CABP0330
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around Y139 of human Phospho-CFL1-Y139 (NP_005498.1).
The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.
Purification Method:
Affinity purification
Gene ID:
1072
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-CFL1-Y139 Rabbit pAb (CABP0330) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
