The Phospho-CHUK (S176) Polyclonal Antibody (CABP0505) is a valuable tool for researchers studying the CHUK protein, a key regulator of the NF-κB signaling pathway. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the phosphorylated form of CHUK at serine 176, this antibody allows for precise detection and analysis of CHUK activation in various cell types.CHUK, also known as inhibitor of nuclear factor kappa-B kinase subunit alpha, plays a crucial role in the regulation of immune and inflammatory responses. Activation of CHUK leads to the phosphorylation and subsequent degradation of inhibitor proteins, resulting in the release of NF-κB and the initiation of pro-inflammatory gene expression.
Understanding the phosphorylation status of CHUK at serine 176 is important for deciphering the mechanisms underlying NF-κB activation and its implications in diseases such as cancer, autoimmune disorders, and inflammatory conditions.Overall, the Phospho-CHUK (S176) Polyclonal Antibody is a reliable tool for researchers investigating the function of CHUK and its involvement in immune and inflammatory pathways. Its specificity and sensitivity make it an ideal choice for studies in immunology, cancer research, and drug development targeting the NF-κB signaling cascade.
Product Name:
Phospho-IKKα-S176 Rabbit Polyclonal Antibody
SKU:
CABP0505
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S176 of human IKKα (NP_001269.3).
This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.
Purification Method:
Affinity purification
Gene ID:
1147
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells, using Phospho-IKKα-S176 Rabbit pAb (CABP0505) at 1:1000 dilution.C6 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
