The Phospho-Chk2 (T68) Monoclonal Antibody (CABP1407) is a valuable tool for researchers studying the Chk2 protein kinase, a key player in the DNA damage response pathway. This antibody, produced using monoclonal technology, is highly specific for detecting phosphorylation at threonine 68 of Chk2. It has been validated for use in techniques such as Western blotting and immunofluorescence, providing researchers with a reliable method for studying the activation status of Chk2 in various cell types.Chk2 is known to be activated in response to DNA damage, leading to cell cycle arrest and DNA repair. Phosphorylation at threonine 68 is a crucial step in this activation process, making the Phospho-Chk2 (T68) Monoclonal Antibody an essential tool for researchers investigating DNA damage signaling pathways.
By understanding the regulation of Chk2 phosphorylation, scientists can gain insights into mechanisms of genomic stability and potential targets for cancer therapy development.Overall, the Phospho-Chk2 (T68) Monoclonal Antibody (CABP1407) is a valuable resource for researchers interested in unraveling the complexities of the DNA damage response and its implications for cancer and other diseases. Its high specificity and reliability make it a trusted tool for studying Chk2 activation and its role in maintaining genomic integrity.
Product Name:
Phospho-Chk2-T68 Monoclonal Antibody
SKU:
CABP1407
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T68 of human Chk2 (NP_009125.1).
In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
11200
Clone Number:
ARC58395
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Immunohistochemistry analysis of paraffin-embedded Human cervix cancer using Phospho-Chk2-T68 Rabbit mAb (CABP1407) at dilution of 1:1000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
