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Phospho-Chk2-S516 Rabbit Polyclonal Antibody (CABP0604)

SKU:
CABP0604
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
$503
Frequently bought together:

Description

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Phospho-Chk2-S516 Rabbit Polyclonal Antibody (CABP0604)

The Phospho-Chk2 (S516) Rabbit Polyclonal Antibody (CABP0604) is a valuable tool for researchers studying the DNA damage response pathway. This antibody specifically recognizes and binds to the phosphorylated form of Chk2 at serine 516, enabling the detection and analysis of this important protein in various cell types.Chk2 is a key player in the DNA damage response, acting as a checkpoint kinase to regulate cell cycle progression and cell survival in response to DNA damage. Phosphorylation of Chk2 at serine 516 is a critical event in its activation and signaling cascade in response to DNA double-strand breaks.

This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. It is an essential tool for researchers investigating the role of Chk2 in DNA damage repair, cell cycle regulation, and cancer biology. Its specificity for the phosphorylated form of Chk2 at serine 516 makes it a valuable resource for studies aiming to understand the molecular mechanisms underlying DNA damage response and potential therapeutic targets in cancer treatment.

Product Name:Phospho-Chk2-S516 Rabbit Polyclonal Antibody
SKU:CABP0604
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around S516 of human Chk2 (NP_009125.1).
Sequence:QPST S
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425; Phospho-Chk2-S516
Positive Sample:NIH/3T3
Conjugate:Unconjugated
Cellular Localization:Nucleus, Nucleus, PML body, nucleoplasm.
Calculated MW:61kDa
Observed MW:62kDa

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene.

Purification Method:Affinity purification
Gene ID:11200
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of NIH/3T3 cells, using Phospho-Chk2-S516 pAb (CABP0604) at 1:1000 dilution or Chk2 antibody (CAB0466).NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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