The Phospho-Chk1 (S296) Rabbit Monoclonal Antibody (CABP1047) is a valuable tool for researchers studying cell cycle regulation and DNA damage response pathways. This highly specific antibody is raised in rabbits and is specifically reactive with phosphorylated Chk1 at serine 296 in human samples. Chk1 is a key regulator of cell cycle checkpoints in response to DNA damage, playing a critical role in maintaining genomic stability and preventing the proliferation of damaged cells. Phosphorylation of Chk1 at serine 296 is an important post-translational modification that modulates its activity in response to DNA damage, making this antibody a valuable tool for researchers studying this regulatory mechanism.Validated for use in Western blot applications, the Phospho-Chk1 (S296) Rabbit Monoclonal Antibody enables detection and analysis of phosphorylated Chk1 in various cell types, providing valuable insights into the signaling pathways involved in DNA damage response.
Its high specificity and sensitivity make it an ideal choice for studies in cancer research, where dysregulation of cell cycle checkpoints and DNA damage response pathways play a crucial role in tumorigenesis.Overall, the Phospho-Chk1 (S296) Rabbit Monoclonal Antibody (CABP1047) offers researchers a powerful tool for investigating the role of Chk1 phosphorylation in cell cycle regulation, DNA damage response, and cancer biology. Its application in Western
Product Name:
Phospho-Chk1-S296 Rabbit Monoclonal Antibody
SKU:
CABP1047
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S296 of human Chk1 (O14757).
The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
1111
Clone Number:
ARC1593
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of 293T cells, using Phospho-Chk1-S296 Rabbit mAb (CABP1047) at 1:1000 dilution.293T cells were treated by UV at room temperature for 15-30 minutes and Doxorubicin (0.5 uM) at 37℃ for 24 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
