The Phospho-CHK1 (S280) Monoclonal Antibody (CABP1444) is a valuable tool for researchers studying the CHK1 protein, a key regulator of cell cycle checkpoints and DNA damage response. This antibody, produced using advanced monoclonal antibody technology, specifically targets the phosphorylated form of CHK1 at Serine 280, a critical site for its activation and function.CHK1 is a crucial protein involved in maintaining genomic stability and promoting cell survival under stress conditions. Phosphorylation at Serine 280 is known to be a key regulatory event that affects CHK1 activity and its role in cell cycle regulation.
The Phospho-CHK1 (S280) Monoclonal Antibody enables researchers to detect and analyze the phosphorylation status of CHK1 in various experimental settings, such as Western blotting and immunofluorescence assays.By targeting the phosphorylated form of CHK1 at Serine 280, researchers can gain insight into the activation status of CHK1 and its involvement in cell cycle control, DNA repair, and response to DNA damage. This antibody is a valuable tool for studying the molecular mechanisms underlying CHK1 function and its potential as a target for cancer therapy and other diseases related to genomic instability.
Product Name:
Phospho-Chk1-S280 Monoclonal Antibody
SKU:
CABP1444
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S280 of human Chk1(NP_001107593.1).
The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
1111
Clone Number:
ARC62579
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of 293T, using Phospho-Chk1-S280 Rabbit mAb (CABP1444) at 1:2000 dilution.293T cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.
