The Phospho-Chk1 (S280) Monoclonal Antibody (CABP1426) is a key tool for researchers studying the Chk1 protein, a critical component in cell cycle regulation and DNA damage response pathways. This antibody, generated using state-of-the-art monoclonal technology, specifically recognizes the phosphorylated form of Chk1 at serine 280, allowing for precise detection and analysis in various experimental settings.Chk1 is a key player in ensuring genome stability and cell survival under stress conditions, making it an attractive target for cancer research and drug development.
Phosphorylation of Chk1 at serine 280 has been implicated in DNA damage response, cell cycle checkpoints, and apoptosis, highlighting its importance in maintaining genomic integrity.By using the Phospho-Chk1 (S280) Monoclonal Antibody, researchers can delve deeper into the intricate mechanisms by which Chk1 regulates cell cycle progression and DNA repair processes. This antibody's high specificity and sensitivity make it an invaluable tool for exploring Chk1 biology and its potential as a therapeutic target in cancer treatment strategies.
Product Name:
Phospho-Chk1-S280 Monoclonal Antibody
SKU:
CABP1426
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A phospho synthetic peptide corresponding to residues surrounding S280 of Human Chk1.
The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
1111
Clone Number:
ARC3123
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of 293T, using Phospho-Chk1 (S280) Rabbit mAb (CABP1426) at 1:500 dilution.293T cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
