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Phospho-Chk1-S280 Monoclonal Antibody (CABP1426)

SKU:
CABP1426
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
€139 - €609
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-Chk1-S280 Monoclonal Antibody
SKU:CABP1426
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A phospho synthetic peptide corresponding to residues surrounding S280 of Human Chk1.
Tested Applications:WB ELISA
Recommended Dilution:WB,1:200 - 1:500
Synonyms:CHK1; Phospho-Chk1-S280
Positive Sample:293T+Calyculin A
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus, Centrosome, Cytoskeleton, Microtubule organizing center.
Calculated MW:54kDa
Observed MW:54kDa

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.

Purification Method:Affinity purification
Gene ID:1111
Clone Number:ARC3123
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of 293T, using Phospho-Chk1 (S280) Rabbit mAb (CABP1426) at 1:500 dilution.293T cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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