The Phospho-C/EBPβ (T235) Rabbit Polyclonal Antibody (CABP1055) is a valuable tool for researchers studying the phosphorylation of the CCAAT/enhancer-binding protein beta (C/EBPβ) transcription factor. This antibody, raised in rabbits, specifically detects the phosphorylated form of C/EBPβ at threonine 235 in human samples and is validated for use in Western blot applications. C/EBPβ is a key regulator of gene expression involved in various cellular processes, including differentiation, proliferation, and immune response. Phosphorylation at threonine 235 is known to impact the transcriptional activity of C/EBPβ and regulate its function in different cellular contexts.
The ability to specifically detect and quantify phosphorylated C/EBPβ at threonine 235 provides valuable insights into the signaling pathways and regulatory mechanisms involving this transcription factor. This antibody is ideal for researchers studying signal transduction, gene regulation, and cell differentiation in the fields of immunology, cancer research, and developmental biology. By understanding the phosphorylation status of C/EBPβ, researchers can uncover new therapeutic targets and potential treatment strategies for diseases influenced by this transcription factor.
Product Name:
Phospho-C/EBPB-T235 Rabbit Polyclonal Antibody
SKU:
CABP1055
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around T235 of human C/EBPB (NP_005185.2).
This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions.
Purification Method:
Affinity purification
Gene ID:
1051
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of NIH/3T3, using Phospho-C/EBPB-T235 antibody (CABP1055) at 1:1000 dilution.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.NIH/3T3 cells were treated by Serum-starvation overnight at 37℃.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
