The Phospho-CDKN1B (T187) Polyclonal Antibody (CABP0328) is a valuable tool for researchers studying the phosphorylation of CDKN1B, a key protein involved in cell cycle regulation and tumor suppression. This antibody, raised in rabbits, is highly specific to the phosphorylated form of CDKN1B at threonine 187 and is validated for use in Western blot and immunohistochemistry applications.By detecting and quantifying the phosphorylated form of CDKN1B, researchers can gain insights into the signaling pathways that regulate cell cycle progression and cell growth. The phosphorylation of CDKN1B at threonine 187 is known to regulate its stability and activity, making this antibody essential for studying the functional impact of this post-translational modification.
With its high specificity and sensitivity to phospho-CDKN1B (T187), this antibody is a valuable tool for cancer research, as dysregulation of CDKN1B phosphorylation has been implicated in various cancer types. By understanding the role of phosphorylated CDKN1B in tumorigenesis, researchers can potentially develop targeted therapies that restore normal cell cycle control and inhibit tumor growth. This antibody is a crucial resource for investigating the molecular mechanisms underlying cancer development and progression.
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4).
Purification Method:
Affinity purification
Gene ID:
1027
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-CDKN1B/p27KIP1-T187 Rabbit pAb (CABP0328).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.