The Phospho-CDK9 (T186) Polyclonal Antibody (CABP0810) is a valuable tool for researchers studying the regulation of CDK9 protein activity. This antibody, generated in rabbits, specifically recognizes the phosphorylated form of CDK9 at threonine 186, a critical residue involved in the control of transcriptional elongation.CDK9 is a key regulator of gene expression and plays a crucial role in various cellular processes, including cell cycle progression and transcriptional regulation. Phosphorylation of CDK9 at T186 is known to affect its kinase activity and substrate specificity, making it an important target for investigating the molecular mechanisms underlying transcriptional regulation.The Phospho-CDK9 (T186) Polyclonal Antibody is extensively validated for use in Western blot applications with human samples, providing researchers with a reliable tool for detecting and quantifying phospho-CDK9 levels in various biological samples.
Its high specificity and sensitivity make it an ideal choice for studies focusing on transcriptional control, cell cycle regulation, and the development of novel cancer therapies targeting CDK9 activity.Overall, the Phospho-CDK9 (T186) Polyclonal Antibody is an essential reagent for researchers interested in understanding the function of CDK9 and its role in cellular processes, offering valuable insights into the complex regulatory networks governing gene expression and transcriptional elongation.
Product Name:
Phospho-CDK9-T186 Rabbit Polyclonal Antibody
SKU:
CABP0810
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T186 of human CDK9 (NP_001252.1).
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.
Purification Method:
Affinity purification
Gene ID:
1025
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-CDK9-T186 Rabbit pAb (CABP0810) at 1:1000 dilution.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
