The Phospho-CDK6 (Y13) Polyclonal Antibody (CABP0326) is a valuable tool for researchers studying CDK6, a key player in cell cycle regulation and cancer development. This antibody, raised in rabbits, is highly specific to the Y13 phosphorylation site on CDK6 and is validated for use in Western blot and immunohistochemistry applications.CDK6 is a cyclin-dependent kinase that promotes cell cycle progression by phosphorylating key proteins involved in cell division. Phosphorylation of CDK6 at the Y13 site is known to regulate its activity and downstream signaling pathways, making it a crucial target for understanding the role of CDK6 in cancer and other diseases.
With its high specificity and sensitivity, the Phospho-CDK6 (Y13) Polyclonal Antibody enables researchers to study the phosphorylation status of CDK6 in various cell types and tissues. By elucidating the mechanisms by which CDK6 is regulated, this antibody can help advance our understanding of cell cycle control and potentially identify new therapeutic targets for cancer treatment.
Product Name:
Phospho-CDK6-Y13 Rabbit Polyclonal Antibody
SKU:
CABP0326
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around Y13 of human CDK6 (NP_001250.1).
Sequence:
QQYE C
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB,1:500 - 1:2000 IF/ICC,1:100 - 1:200
Synonyms:
MCPH12; PLSTIRE; Phospho-CDK6-Y13
Positive Sample:
293
Conjugate:
Unconjugated
Cellular Localization:
Cell projection, Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, ruffle.
Calculated MW:
37kDa
Observed MW:
37kDa
The protein encoded by this gene is a member of the CMGC family of serine/threonine protein kinases. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Altered expression of this gene has been observed in multiple human cancers. A mutation in this gene resulting in reduced cell proliferation, and impaired cell motility and polarity, and has been identified in patients with primary microcephaly.
Purification Method:
Affinity purification
Gene ID:
1021
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293 cells using Phospho-CDK6-Y13 Rabbit pAb (CABP0326).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
