The Phospho-CDK4 (T172) Polyclonal Antibody (CABP0593) is a critical tool for researchers studying cell cycle regulation and cancer cell proliferation. This rabbit-derived antibody specifically recognizes phosphorylated CDK4 at threonine 172, a key site involved in the activation of CDK4 and subsequent cell cycle progression.Validated for use in Western blot applications, this antibody is highly reactive with human samples, enabling precise detection and analysis of phospho-CDK4 levels in various cell types. Its specificity for phosphorylated CDK4 makes it an ideal tool for investigating the role of this protein kinase in cancer development and progression.
Phospho-CDK4 is a crucial regulator of cell cycle progression, with dysregulation of its activity implicated in various types of cancer. By targeting the phosphorylation state of CDK4, researchers can gain insights into the mechanisms driving uncontrolled cell proliferation and potentially identify novel therapeutic targets for cancer treatment.Overall, the Phospho-CDK4 (T172) Polyclonal Antibody (CABP0593) offers a valuable resource for scientists studying cell cycle biology, cancer research, and potential therapeutic interventions aimed at targeting CDK4 activity in cancer cells.
Product Name:
Phospho-CDK4-T172 Rabbit Polyclonal Antibody
SKU:
CABP0593
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T172 of human CDK4 (NP_000066.1).
Sequence:
ALTP VV
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
CMM3; PSK-J3; Phospho-CDK4-T172
Positive Sample:
NIH/3T3
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Membrane, Nucleus.
Calculated MW:
34kDa
Observed MW:
33kDa
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.
Purification Method:
Affinity purification
Gene ID:
1019
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-CDK4-T172 Rabbit pAb (CAB0366).NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
