The Phospho-CDK2 (Y15) Rabbit Monoclonal Antibody (CABP1005) is a vital tool for researchers studying cell cycle regulation and cancer development. This antibody specifically targets phosphorylated CDK2 at Tyrosine 15, a key regulatory site that controls the activity of this cyclin-dependent kinase. CDK2 is a crucial player in cell cycle progression, regulating entry into S phase and promoting cell division. Phosphorylation at Y15 inhibits CDK2 activity, preventing premature cell cycle progression and ensuring proper cell division. Dysregulation of CDK2 activity, often seen in cancer cells, can lead to uncontrolled cell proliferation and tumor formation.This antibody, generated in rabbits, is highly specific and sensitive, making it ideal for detecting changes in CDK2 phosphorylation in various cell types and experimental conditions.
Its validation for use in Western blot applications ensures reliable and accurate results in research studies focused on cell cycle regulation, cancer biology, and drug discovery.By targeting phospho-CDK2 (Y15), researchers can gain valuable insights into the mechanisms underlying cell cycle control and cancer progression. Understanding the role of CDK2 phosphorylation in disease states may lead to the development of novel therapeutic strategies aimed at targeting this key regulator of cell division.
Product Name:
Phospho-CDK2-Y15 Rabbit Monoclonal Antibody
SKU:
CABP1005
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
A synthetic phosphorylated peptide around Y15 of human CDK2 (NP_001789.2).
Sequence:
GTYG V
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
CDKN2; p33(CDK2); Phospho-CDK2-Y15
Positive Sample:
NIH/3T3 cells were treated by Hydroxyurea (4 Mm) at 37℃ for 20 hours or treated by Nocodazole (100 ng/mL) at 37℃ for 24 hours.
This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1017
Clone Number:
ARC1550
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-CDK2-Y15 Rabbit mAb (CABP1005) at 1:1000 dilution.NIH/3T3 cells were treated by Hydroxyurea (4 Mm) at 37℃ for 20 hours or treated by Nocodazole (100 ng/mL) at 37℃ for 24 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
