The Phospho-CDK1 (Y15) Polyclonal Antibody (CABP0016) is a vital tool for researchers studying cell cycle regulation and cell division processes. This antibody, raised in rabbits, is highly specific and reacts with human and mouse samples, making it versatile for use in a variety of experimental settings.Phospho-CDK1 (Y15) is a phosphorylated form of the cyclin-dependent kinase 1 (CDK1) protein, which plays a crucial role in regulating the progression of the cell cycle. Phosphorylation of CDK1 at the Y15 residue is known to inhibit its activity, thereby controlling cell cycle progression and ensuring proper cell division.
This antibody is validated for use in Western blot applications, allowing for sensitive detection and analysis of Phospho-CDK1 (Y15) levels in various cell types and tissues. By enabling researchers to study the phosphorylation status of CDK1, this antibody is essential for investigating the mechanisms underlying cell cycle regulation and identifying potential targets for therapeutic intervention in diseases such as cancer.
Product Name:
Phospho-CDK1-Y15 Rabbit Polyclonal Antibody
SKU:
CABP0016
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around Y15 of human CDK1 (NP_001777.1).
Sequence:
GTYG V
Tested Applications:
WBIHC-PIF/ICCIPELISA
Recommended Dilution:
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
CDC2; CDC28A; P34CDC2; Phospho-CDK1-Y15
Positive Sample:
HT-29,HeLa,NIH/3T3,C6
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Mitochondrion, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle.
Calculated MW:
34kDa
Observed MW:
34kDa
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
983
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-CDK1-Y15 Rabbit pAb (CAB0220).HeLa cellls were treated by nocodazole (50 ng/mL) at 37℃ for 20 hours or Hydroxyurea (4 mM) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
