The Phospho-CDK1 (Y15) Monoclonal Antibody is a valuable tool for researchers investigating the role of CDK1, a key regulator of cell cycle progression, in phosphorylation at tyrosine 15. This antibody, produced through monoclonal technology, is highly specific to human samples and is validated for use in various applications, including Western blot and immunofluorescence.CDK1, a member of the cyclin-dependent kinase family, plays a crucial role in cell cycle control by regulating the transition from G2 phase to mitosis. Phosphorylation of CDK1 at tyrosine 15 is known to be a key regulatory step in controlling its activity.
This antibody enables detection and analysis of phosphorylated CDK1 in different cell types, providing valuable insight into cell cycle regulation and potential therapeutic targets for diseases such as cancer.The Phospho-CDK1 (Y15) Monoclonal Antibody is an essential tool for researchers studying cell cycle regulation, cancer biology, and drug development. Its specificity and reliability make it a valuable asset in advancing our understanding of CDK1 function and its implications in various disease states.
Product Name:
Phospho-CDK1-Y15 Monoclonal Antibody
SKU:
CABP1350
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around Y15 of human CDK1(NP_001777.1).
Sequence:
GTYG V
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:1000 - 1:5000
Synonyms:
CDC2; CDC28A; P34CDC2; Phospho-CDK1-Y15
Positive Sample:
HeLa+Hydroxyurea
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Mitochondrion, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle.
Calculated MW:
34kDa
Observed MW:
34kDa
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
983
Clone Number:
ARC55443
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Phospho-CDK1-Y15 antibody (CABP1350) at1:2000 dilution.HeLa cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
