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Phospho-CDK1-Y15 Monoclonal Antibody (CABP1350)

SKU:
CABP1350
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
€139 - €609
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-CDK1-Y15 Monoclonal Antibody
SKU:CABP1350
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around Y15 of human CDK1(NP_001777.1).
Sequence:GTYG V
Tested Applications:WB ELISA
Recommended Dilution:WB,1:1000 - 1:5000
Synonyms:CDC2; CDC28A; P34CDC2; Phospho-CDK1-Y15
Positive Sample:HeLa+Hydroxyurea
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Mitochondrion, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle.
Calculated MW:34kDa
Observed MW:34kDa

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Purification Method:Affinity purification
Gene ID:983
Clone Number:ARC55443
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates, using Phospho-CDK1-Y15 antibody (CABP1350) at1:2000 dilution.HeLa cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.

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