The Phospho-CDK1 (T161) Polyclonal Antibody (CABP0324) is a valuable tool for researchers studying cell cycle regulation and mitosis. This antibody, raised in rabbits, specifically targets the phosphorylated form of CDK1 at threonine 161, a key regulatory site for the activity of this important cell cycle regulator.CDK1 is a critical kinase involved in the control of cell division and plays a vital role in regulating the G2/M transition. Phosphorylation of CDK1 at threonine 161 is known to be essential for the activation of the kinase and the onset of mitosis. This antibody enables researchers to detect and analyze the phosphorylation status of CDK1 in various cell types, providing valuable insights into the mechanisms controlling cell cycle progression.
The Phospho-CDK1 (T161) Polyclonal Antibody is validated for use in Western blot applications and has been tested for reactivity with human samples. Its high specificity and sensitivity make it an ideal tool for studying the role of CDK1 phosphorylation in cell cycle regulation, making it a valuable resource for research in areas such as cancer biology, developmental biology, and cell signaling pathways.
Product Name:
Phospho-CDK1-T161 Rabbit Polyclonal Antibody
SKU:
CABP0324
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T161 of human CDK1 (NP_001777.1).
Sequence:
VYTH E
Tested Applications:
WBIHC-PIF/ICCIPELISA
Recommended Dilution:
WB,1:100 - 1:500 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
CDC2; CDC28A; P34CDC2; Phospho-CDK1-T161
Positive Sample:
HeLa
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Mitochondrion, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle.
Calculated MW:
34kDa
Observed MW:
34kDa
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
983
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-CDK1-T161 Rabbit pAb (CABP0324) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
