null

Phospho-CDK1-T161 Rabbit Polyclonal Antibody (CABP0324)

SKU:
CABP0324
Product Type:
Antibody
Applications:
WB
IHC
IF
IP
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death
  • Cell Death Antibodies 2 Anti-Phospho-CDK1-T161 Antibody CABP0324
  • Cell Death Antibodies 2 Anti-Phospho-CDK1-T161 Antibody CABP0324
  • Cell Death Antibodies 2 Anti-Phospho-CDK1-T161 Antibody CABP0324
€419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-CDK1-T161 Rabbit Polyclonal Antibody
SKU:CABP0324
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around T161 of human CDK1 (NP_001777.1).
Sequence:VYTH E
Tested Applications:WB IHC-P IF/ICC IP ELISA
Recommended Dilution:WB,1:100 - 1:500 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:CDC2; CDC28A; P34CDC2; Phospho-CDK1-T161
Positive Sample:HeLa
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Mitochondrion, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle.
Calculated MW:34kDa
Observed MW:34kDa

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Purification Method:Affinity purification
Gene ID:983
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from HeLa cells, using Phospho-CDK1-T161 Rabbit pAb (CABP0324) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Related Products