The Phospho-CDK1 (T14) Polyclonal Antibody (CABP0015) is a valuable tool for researchers studying cell cycle regulation and mitosis. CDK1, also known as cyclin-dependent kinase 1, is a key player in cell division and is regulated by phosphorylation at threonine 14 (T14) during mitosis. This antibody, raised in rabbits, is highly specific for the phosphorylated form of CDK1 and is validated for use in Western blot applications.The Phospho-CDK1 (T14) antibody binds specifically to the phosphorylated form of CDK1, allowing for precise detection and analysis of CDK1 activity in various cell types. This antibody is essential for studying the role of CDK1 phosphorylation in cell cycle progression and mitotic events, making it an ideal tool for research in cell biology, cancer biology, and drug development.
Understanding the regulation of CDK1 phosphorylation at T14 is crucial for unraveling the complex mechanisms that control cell division and proliferation. This antibody provides researchers with a reliable and sensitive tool for investigating the role of CDK1 in cell cycle progression, making it a valuable asset for studies aimed at uncovering the molecular mechanisms underlying cancer and other proliferative diseases.
Product Name:
Phospho-CDK1-T14 Rabbit Polyclonal Antibody
SKU:
CABP0015
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T14 of human CDK1 (NP_001777.1).
Sequence:
EGTY G
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
CDC2; CDC28A; P34CDC2; Phospho-CDK1-T14
Positive Sample:
HeLa
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Mitochondrion, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle.
Calculated MW:
34kDa
Observed MW:
34kDa
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
983
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-CDK1-T14 Rabbit pAb (CABP0015) at 1:500 dilution.HeLa cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
