The Phospho-CDK1/2 (T14) Rabbit Monoclonal Antibody (CABP1001) is a valuable tool for researchers studying cell cycle regulation and phosphorylation events. This antibody specifically detects the phosphorylated forms of CDK1 and CDK2 at threonine 14, key sites that regulate the activity of these critical cell cycle regulators.With its high reactivity with human samples and validation for use in Western blot applications, this antibody enables precise detection and analysis of phospho-CDK1/2 in various cell types. Its ability to target the phosphorylated forms of CDK1 and CDK2 makes it an essential tool for investigating cell cycle progression, DNA damage response, and cancer biology.
The phosphorylation of CDK1 and CDK2 at threonine 14 is a tightly regulated process essential for proper cell cycle control. Dysregulation of these phosphorylation events has been linked to various diseases, including cancer. By using this antibody, researchers can gain insights into the role of phospho-CDK1/2 in cell cycle regulation and potentially identify new therapeutic targets for treating cancer and other diseases.Overall, the Phospho-CDK1/2 (T14) Rabbit Monoclonal Antibody (CABP1001) is a reliable and specific tool for studying the phosphorylation status of CDK1 and CDK2 in research areas such as cell biology, cancer biology, and drug
Product Name:
Phospho-Cdk1/2-T14 Rabbit Monoclonal Antibody
SKU:
CABP1001
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T14 of human Cdk1/2CDK1 (P06493).
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Purification Method:
Affinity purification
Gene ID:
983/1017
Clone Number:
ARC1546
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Phospho-Cdk1/2-T14 Rabbit mAb (CABP1001) at 1:1000 dilution.HeLa cells and NIH/3T3 cells and C6 cells were treated by Hydroxyurea (4 Mm) at 37℃ for 20 hours or treated by Nocodazole (100 ng/mL) at 37℃ for 24 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
