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Phospho-Cdk1/2-T14 Rabbit Monoclonal Antibody (CABP1001)

SKU:
CABP1001
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
€419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-Cdk1/2-T14 Rabbit Monoclonal Antibody
SKU:CABP1001
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around T14 of human Cdk1/2CDK1 (P06493).
Sequence:EGTY G
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:CDC2; CDC28A; P34CDC2; Phospho-Cdk1/2-T14
Positive Sample:HeLa,HeLa+Hydroxyurea,HeLa+Nocodazole,NIH/3T3,NIH/3T3+Hydroxyurea,NIH/3T3+Nocodazole,C6,C6+Hydroxyurea,C6+Nocodazole
Conjugate:Unconjugated
Cellular Localization:Centrosome, Cytoplasm, Cytosol, Extracellular exosome, Mitochondrial matrix, Mitochondrion, Mitotic spindle, Nucleoplasm, Nucleus, Spindle microtubule
Calculated MW:34kDa
Observed MW:34kDa

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Purification Method:Affinity purification
Gene ID:983/1017
Clone Number:ARC1546
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using Phospho-Cdk1/2-T14 Rabbit mAb (CABP1001) at 1:1000 dilution.HeLa cells and NIH/3T3 cells and C6 cells were treated by Hydroxyurea (4 Mm) at 37℃ for 20 hours or treated by Nocodazole (100 ng/mL) at 37℃ for 24 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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