The Phospho-CDC37 (S13) Rabbit Monoclonal Antibody (CABP1141) is a valuable tool for researchers studying the phosphorylation of CDC37 at serine 13, a key regulatory event in cellular signaling pathways. This antibody, meticulously developed using rabbit monoclonal technology, exhibits high specificity and sensitivity towards human samples, making it suitable for a range of applications including Western blotting.CDC37 is a molecular chaperone that plays a crucial role in the stabilization and activation of client protein kinases involved in cell growth and proliferation. Phosphorylation of CDC37 at serine 13 is known to modulate its interaction with client kinases, thereby influencing their activity and downstream signaling cascades.
By utilizing the Phospho-CDC37 (S13) Rabbit Monoclonal Antibody, researchers can gain insights into the regulatory mechanisms governing kinase function and potentially identify new therapeutic targets for cancer and other diseases.This antibody is an essential addition to any laboratory conducting research in the fields of cell signaling, protein phosphorylation, and kinase biology. Its high specificity and performance in Western blot applications make it a reliable tool for uncovering the intricate signaling networks that drive cellular processes and disease progression.
Product Name:
Phospho-CDC37-S13 Rabbit Monoclonal Antibody
SKU:
CABP1141
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S13 of human CDC37 (Q16543).
Sequence:
EVSD D
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
P50CDC37; Phospho-CDC37-S13
Positive Sample:
293T,NIH/3T3,C6
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm.
Calculated MW:
44kDa
Observed MW:
50kDa
The protein encoded by this gene is highly similar to Cdc 37, a cell division cycle control protein of Sacchromyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases.
Purification Method:
Affinity purification
Gene ID:
11140
Clone Number:
ARC1602
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Phospho-CDC37-S13 Rabbit mAb (CABP1141) at 1:1000 dilution.Both 293T cells and NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
