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Phospho-cdc25C-S216 Rabbit Polyclonal Antibody (CABP0577)

SKU:
CABP0577
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
$503
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-cdc25C-S216 Rabbit Polyclonal Antibody
SKU:CABP0577
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around S216 of human cdc25C (NP_001781.2).
Sequence:SPSM P
Tested Applications:WB IHC-P ELISA
Recommended Dilution:WB,1:100 - 1:500 IHC-P,1:50 - 1:200
Synonyms:CDC25; PPP1R60; Phospho-cdc25C-S216
Positive Sample:HT-29+λ-PP
Conjugate:Unconjugated
Cellular Localization:Nucleus.
Calculated MW:53kDa
Observed MW:60kDa

This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described.

Purification Method:Affinity purification
Gene ID:995
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from HT-29 cells, using Phospho-cdc25C-S216 Rabbit pAb (CABP0577) at 1:400 dilution.HT-29 cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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