The Phospho-CDC25B (Ser149) Antibody (PAC024346) is a crucial tool for researchers studying cell cycle regulation and signaling pathways. CDC25B is a key regulator of cell division, specifically the G2/M transition, and its phosphorylation at Ser149 is important for its activity. This antibody, produced in rabbits, is highly specific for phosphorylated CDC25B at Ser149 and is validated for use in Western blot applications.By targeting the phosphorylated form of CDC25B, researchers can investigate the role of this protein in cell cycle progression and its potential as a therapeutic target in cancer and other diseases characterized by dysregulated cell division.
The antibody's specificity for the Ser149 phosphorylation site ensures accurate detection and analysis, making it a valuable tool for advancing our understanding of cell cycle control mechanisms.
Antibody Name:
Phospho-Cdc25b (Ser149) Antibody (PACO24346)
Antibody SKU:
PACO24346
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:1000
Species Reactivity:
Mouse, Rat
Immunogen:
Peptide sequence around phosphorylation site of Serine 149 (F-R-S(p)-L-P) derived from Mouse Cdc25B.
Form:
Liquid
Storage Buffer:
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from 3T3 cells untreated(lane 1) or treated with Hydroxyurea(lane 2) using Cdc25B(Phospho-Ser149) Antibody.
Western blot analysis of extracts from HT29 cells, treated with PMA or calf intestinal phosphatase (CIP), using Cdc25B (Phospho-Ser149) Antibody.
Background:
Cdc25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.
Li G, et al. (2010) Retrovirology.7:59. Wang Z,et al. (2010) BMC Cancer.10:233. Lucci MA,et al. (2010) Cell Oncol.32(5-6):361-72.
