The Phospho-CCNE1 (T395) Polyclonal Antibody (CABP0013) is a valuable tool for researchers studying cell cycle regulation and cancer biology. This antibody, produced in rabbits, is highly specific for detecting phosphorylated threonine 395 on the CCNE1 protein. Validated for use in Western blot applications, it allows for precise detection and analysis of CCNE1 phosphorylation in various cell types.CCNE1 is a key regulator of the cell cycle, specifically involved in the G1/S transition. Phosphorylation of CCNE1 at threonine 395 has been linked to cancer progression and poor prognosis in some malignancies.
This antibody enables researchers to investigate the role of CCNE1 phosphorylation in cancer development and potentially identify novel therapeutic targets.By utilizing the Phospho-CCNE1 (T395) Polyclonal Antibody (CABP0013), researchers can gain insights into the molecular mechanisms underlying cancer progression and potentially pave the way for the development of targeted therapies aimed at disrupting CCNE1 phosphorylation. This antibody is a valuable tool for studies in cancer biology, cell signaling, and drug development.
Product Name:
Phospho-Cyclin E1-T395 Rabbit Polyclonal Antibody
SKU:
CABP0013
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T395 of human Cyclin E1 (NP_001229.1).
Sequence:
LLTP P
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200
Synonyms:
CCNE; pCCNE1; Phospho-Cyclin E1-T395
Positive Sample:
HepG2,HT-29
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
47kDa
Observed MW:
55kDa
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB.
Purification Method:
Affinity purification
Gene ID:
898
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Phospho-Cyclin E1-T395 antibody (CABP0013).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
