The Phospho-CBX3 (S83) Polyclonal Antibody (CABP0801) is a valuable tool for researchers studying CBX3, a protein involved in chromatin organization and gene regulation. This antibody, raised in rabbits, specifically binds to CBX3 phosphorylated at serine 83 and is validated for use in various applications, including Western blotting and immunohistochemistry.CBX3, also known as HP1γ, plays a critical role in maintaining chromatin structure and gene expression patterns. Phosphorylation of CBX3 at serine 83 has been linked to changes in its function, suggesting a role in regulating gene transcription.
Research on the phosphorylation status of CBX3 can provide insights into its role in chromatin dynamics and cellular processes.The Phospho-CBX3 (S83) Polyclonal Antibody is highly specific to the phosphorylated form of CBX3, enabling researchers to analyze its expression and localization in various cell types and tissues. This antibody is an essential tool for studies investigating the role of CBX3 phosphorylation in cellular processes, making it suitable for research in fields such as epigenetics, gene regulation, and cancer biology.
At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
11335
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-HP1 gamma/CBX3-S83 pAb (CABP0801) at 1:1000 dilution or HP1 gamma/CBX3 antibody (CAB2248).HeLa cells were treated by Paclitaxel (100 nM/mL) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
