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Phospho-CBL-Y700 Rabbit Polyclonal Antibody (CABP0780)

SKU:
CABP0780
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction
€419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-CBL-Y700 Rabbit Polyclonal Antibody
SKU:CABP0780
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around Y700 of human CBL (NP_005179.2).
Sequence:TEYM T
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:CBL2; NSLL; C-CBL; RNF55; FRA11B; Phospho-CBL-Y700
Positive Sample:Jurkat
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm.
Calculated MW:100kDa
Observed MW:120kDa

This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia, and expansion of CGG repeats in the 5' UTR has been associated with Jacobsen syndrome. Mutations in this gene are also the cause of Noonan syndrome-like disorder.

Purification Method:Affinity purification
Gene ID:867
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from Jurkat cells, using Phospho-CBL-Y700 Rabbit pAb (CABP0780) at 1:2000 dilution or CBL antibody (CAB0732). Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

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