The Phospho-Catenin (S552) Monoclonal Antibody is a valuable tool for researchers studying the role of beta-catenin in cell signaling pathways. This antibody specifically detects the phosphorylated form of beta-catenin at Serine 552, a key site involved in its regulation and function.Beta-catenin is a crucial component of the Wnt signaling pathway, which has been implicated in various diseases including cancer and developmental disorders. Phosphorylation of beta-catenin at Serine 552 has been shown to affect its stability and activity, making it an important target for investigation.
This monoclonal antibody, produced in mice, is highly specific for the phosphorylated form of beta-catenin at Serine 552 and has been validated for use in various research applications such as immunohistochemistry and Western blotting. Its ability to detect this specific phosphorylation site allows for precise analysis of beta-catenin signaling in different cellular contexts, providing valuable insights into its role in disease progression and potential therapeutic targets.
Product Name:
Phospho-β-Catenin-S552 Monoclonal Antibody
SKU:
CABP1315
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around S552 of human CTNNB1 (NP_001895.1).
Cell junction, Cell membrane, Cytoplasm, Nucleus, adherens junction, centrosome, cytoskeleton, microtubule organizing center, spindle pole.
Calculated MW:
85kDa
Observed MW:
92kDa
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1499
Clone Number:
ARC54001
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using Phospho-β-Catenin-S552 Rabbit mAb (CABP1315) at 2000 dilution.293T cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
