The Phospho-Catenin Beta-1 (T41/S45) Polyclonal Antibody (CABP0336) is a crucial tool for researchers studying the phosphorylation status of the beta-catenin protein. This antibody, developed using rabbit immunization techniques, exhibits strong reactivity with human samples and is specifically designed for Western blot applications. By targeting the phosphorylated sites T41 and S45 on beta-catenin, this antibody allows for precise detection and analysis of protein activation in various cell types.Beta-catenin is a key player in the Wnt signaling pathway, playing a vital role in cell proliferation, differentiation, and tumorigenesis.
The phosphorylation status of beta-catenin at residues T41 and S45 is known to regulate its stability and activity, making it a crucial marker for studying cancer progression and other diseases. By understanding the phosphorylation dynamics of beta-catenin, researchers can gain insights into the molecular mechanisms underlying various cellular processes and diseases, paving the way for the development of targeted therapies.
Cell junction, Cell membrane, Cytoplasm, Nucleus, adherens junction, centrosome, cytoskeleton, microtubule organizing center, spindle pole.
Calculated MW:
85kDa
Observed MW:
85kDa
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1499
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from SW626 cells using Phospho-β-Catenin-T41/S45 Rabbit pAb (CABP0336).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
